Human/Mouse FGF-8 Antibody (2024)

Species Reactivity

Human, Mouse

Specificity

Detects human and mouseFGF-8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh)FGF-5, rhFGF-7, and rhFGF-9 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 47109

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant mouse FGF-8b
Gln23-Arg215
Accession # NP_001159834

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human FGF-8a (Catalog # 4745-F8)
Recombinant Mouse FG-8b (Catalog # 423-F8)
Recombinant Mouse FGF-8c (Catalog # 424-FC)
Recombinant Human FGF-8e (Catalog # 4746-F8)
Recombinant Human FGF-8f (Catalog # 5027-FF)

Immunohistochemistry

8-25 µg/mL

See below

Neutralization

Measured by its ability to neutralize FGF‑8-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND₅₀) is typically 0.25-0.75µg/mL in the presence of 125ng/mL Recombinant Mouse FGF‑8 c Isoform and 0.1µg/mL heparin.

Cell Proliferation Induced by FGF‑8 and Neutralization by Human and Mouse FGF‑8 Antibody. Recombinant Mouse FGF-8 c Isoform (Catalog # 424-FC) stimulates proliferation in the the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse FGF-8 c Isoform (125 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse FGF-8 Monoclonal Antibody (Catalog # MAB323). The ND₅₀ is typically 0.25-0.75 µg/mL in the presence of heparin (0.1 µg/mL).

FGF‑8 in Human Prostate. FGF-8 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human/Mouse FGF-8 Monoclonal Antibody (Catalog # MAB323) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to stromal cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Mouse FGF-8 by Immunohistochemistry Phosphorylation patterns of ERK1/2 (dpERK) enzymes in the anterior neural tube.Whole mount in situ hybridization (ISH) of E9.5 mouse embryo (A,B,B’) and corresponding organotypic neural tissue cultures of mouse E9.5 anterior neural tube (A’,B”,C-E”; ONTCs; [42]) where it shows the maintenance of gene expression profiles such us Fgf8, Tcf4 (A,A’) (used to delimit main brain subdivisions such the diencephalon (D; Tcf4 positive) and the mesencephalic anlage (M; negative staining), Pax6 D-D” (delimiting di-mesencephalic boundary and rhombomere 1–2 limits) and En2 E-E”. In B-B”) are photomicrographs of E9.5 mouse embryo with anti-dpERK Immunohistochemistry (IHC) taken from lateral (B) and caudal (B’) sides and the corresponding IHC in ONTCs. (C) double staining procedure: ISH (in blue) for Fgf8 and IHC for dpERK (dark brown) to localize inside the dpERK domain the position of the IsO, marked by the solid red line. (D-E”) photomicrographs of same ONTCs in which first a whole mount ISH for Pax6 (D) or Tcf4/En2 (E) were made and afterwards IHC against dpERK. (D’,E’ respectively). Dashed lines mark the main transversal (in black) and longitudinal (in red) brain subdivisions. These ONTCs were cut into transversal sections to the isthmic constriction (D”,E”) to proof that indeed dpERK expression reaches diencephalic anlage (D”; see asterisk; rostral is left) and has a wider expansion than En2 expression (E”; see asterisk; rostral is left). anr is anterior neural ridge secondary organizer; ba is branchial arch; IsO is isthmic organizer; os is optic stalk; ov is otic vesicle; r is rhombomere; T is telencephalon, D, diencephalon, M, mesencephalon. Scale bars are 0,5 mm except in D”, E” they are 100 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22792203), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

References

Long Name

Fibroblast Growth Factor 8

Entrez Gene IDs

2253 (Human); 14179 (Mouse); 29349 (Rat)

Alternate Names

AIGF; AIGFKAL6; Androgen-induced growth factor; FGF8; FGF-8; fibroblast growth factor 8 (androgen-induced); fibroblast growth factor 8; HBGF-8; Heparin-binding growth factor 8; MGC149376

COA

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